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Calculate matrix for stretch mode

Usage

.calculate_stretch_matrix(
  bw,
  granges,
  bin_size = 100,
  upstream = 2500,
  downstream = 2500,
  middle = NULL,
  ignore_strand = FALSE,
  default_na = NA_real_,
  scaling = "none"
)

Arguments

bw

BigWigFile object

granges

GRanges object

bin_size

Bin size

upstream

Number of basepairs upstream

downstream

Number of basepairs downstream

middle

Number of base pairs that the middle section has. If not provided, median is used.

ignore_strand

Ignore strand (bool)

default_na

Default value for missing values

scaling

none if no scaling is performed (default), relative if 1x scaling is done (divide by global coverage).

Value

Summary matrix